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Card Consolidation Credit Debt Mahidol University Annual Research Abstracts 2000 153 EFFECT OF TIME INTERVAL BETWEEN SPERM COLLECTION, PREPARATION, AND INSEMINATION ON IN VITRO FERTILIZATION

Credit Union Seesiri Chatasingh¹, Chartchai Srisombut², Anna Wongkularb², Somporn Chinsomboon¹ and Umaporn Udomsubpayakul¹. ¹ Research Center, and ²Departments of Obstetrics & Gynaecology, Ramathibodi Hospital, Faculty of Medicine, Mahidol University, Bangkok, Thailand.

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Merchant Credit Guide Co Key words : sperm preparation time, in vitro fertilization, fertilization rate, cleavage rate

Most cases of low male fertility display an abnormal sperm count or low sperm motility. Even though it takes only one sperm to fertilize an egg, there are many natural barriers in the female reproductive system that keep sperm from reaching the egg. Out of 200 million sperm in an average ejaculate, only 40 reach the vicinity of the eggs. Low sperm count or motility dramatically reduces the chances of the sperm reaching the egg.

Apply Online For Credit Card Introduction : In routine IVF procedure, the timing of semen collection was not definitely specified. It varied from centers to centers. The sooner semen was collected the longer prepared sperm was kept incubating for insemination and vice versa. The effect of this incubation period on human IVF has not been carried out. The aim of this study is to determine the effects of the time interval between sperm collection and preparation, collection and insemination and preparation and insemination on IVF results.

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By Card Credit Debt Debt Guide Materials and methods : Sixty couples who underwent IVF-ET were recruited. They were devided into normal and abnormal sperm parameters, ie group 1 : sperm concentration> 20x10⁶ /ml and % motility> 50 (N=47) and group 2 : sperm concentration < 20x10⁶ / ml or % motility < 50 (N=13). Control ovarian hyperstimulation was achieved by short protocol using GnRH agonist and hMG in every cases. Time interval between semen collection, preparation and insemination were recorded and defined as T₁ for time interval between semen collection and insemination, T₂ for complete sperm preparation and insemination and T₃ for semen collection and sperm preparation. Data were analysed by unpaired t-test to compare means of T₁, T, ₂, T₃, fertilization rate and cleavage rate between groups. Relationships between T₁ or T₂ or T₃ and fertilization rate or cleavage rate were carried out by Pearson and Kendal correlation tests in group 1 and 2, respectively.

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Credit Repair Results : There was no difference in every parameter between the two groups except fertilization rate which was 85.1+16.0 % and 65.0+29.3 % (P=0.037) in group 1 and 2, respectively. In group 1, no correlation was found between T₁ or T₂ or T₃ and fertilization rate or cleavage rate. Neither were the correlations between T₁ or T₂ and fertilization rate and cleavage rate in group 2. Only T₃ expressed inverse correlation between fertilization rate and cleavage rate at r=-0.75 and -0.58 (P=0.001 and 0.018), respectively.

Active Credit Credit Guide Discussion : The finding agreed to that was proposed by Mortimer that sperm need to be separated from the seminal plasma quickly and efficiently. Prolonged time interval between semen collection and preparation especially in abnormal sperm may affect IVF outcomes.

Credit Score (Published in Thai J Obstet Gynaecol, 1999; 11(2):117-22.)

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